Brandon Hoenig

Chytridiomycosis, a disease caused by the pathogenic fungus Batrachochytrium dendrobatidis (Bd), has been linked to amphibian declines across the globe. However, many approaches to detect Bd involve qPCR, a DNA-based method that often requires expensive equipment, dedicated lab spaces, and experience with molecular techniques; limitations which preclude its usage in underfunded laboratories, field situations, or by citizen scientists. Therefore, I am proposing the development of the CRISPR-based system, DETECTR, for the rapid, field ready and cost-effective detection of Bd. As this system requires little training and only body-temperatures to initiate the reactions, DETECTR promises to revolutionize how scientists monitor Bd.